protein purification

Wildwind swvong at
Mon Jul 21 09:14:16 EST 1997


I have extracted protein for 1-D electrophoresis in plant tissue and 
have had no problems in the past. However, recently (in seed tissue) I 
have had severe streaking throughout the entire gels (not just in the 
lanes) following staining, making detection of bands impossible. I asked 
several co-workers who believe it may be polysaccharides in the sample 
buffer. Does anyone have any suggestions on how to fix this problem? It 
would be appreciated.


More information about the Proteins mailing list