protein purification

Wildwind swvong at sciborg.uwaterloo.ca
Mon Jul 21 09:14:16 EST 1997


Hello,

I have extracted protein for 1-D electrophoresis in plant tissue and 
have had no problems in the past. However, recently (in seed tissue) I 
have had severe streaking throughout the entire gels (not just in the 
lanes) following staining, making detection of bands impossible. I asked 
several co-workers who believe it may be polysaccharides in the sample 
buffer. Does anyone have any suggestions on how to fix this problem? It 
would be appreciated.

Thanks



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