Tris/tricine SDS-PAGE

John Tomaszewski tomasz at protein.chem.washington.edu
Sun Jul 27 12:05:39 EST 1997


>Dear everyone, 
>
>I am trying to detect the low molecular weight(8KD) protein using
>Tris-Tricine SDS-PAGE. But when I dried the gel, the gel was easily
>crashed. 

	I run a 16.5% gel (from 29:1 acryl/bis stock) for 6 and 7.5KD 
proteins.  After destaining in 40% MeOH/10% HOAc I give it about an hour 
in 5% MeOH/7% HOAc and it dries just fine under heat and vacuum.

John T.

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**  **  ** ** * **            DEPARTMENT OF CHEMISTRY             **
**  **  ** ** * **        tomasz at protein.chem.washington.edu      **
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**   *****  *****                JOHN TOMASZEWSKI                 ** 
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