how to stop protein degradation?

Randy Willis willis at gandalf.psf.sickkids.on.ca
Thu Mar 6 18:52:48 EST 1997


Thomas Schwartz wrote:
> you could try to express your protein in protease-deficient strains,
> like BL21 for example.


This is not always a guarantee against degradation, as we have had
experience with proteins which are eaten or nibbled by BL21(DE3).  In
one case, we found that we could minimize it with lower temperature
inductions but still required inhibitors upon cell lysis.

Randall C Willis, Researcher
Biochemistry, Hosp for Sick Children
3522-555 University Ave
Toronto, ON
M5G 1X8  CANADA

416-813-5933 (ph)  -5022 (fax)

willis at gandalf.psf.sickkids.on.ca



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