Storage of alkylation reagents
ogsdw at ssa.bris.ac.uk
Mon Mar 10 09:33:35 EST 1997
My antibody only seems to detect its target protein when it is reduced
with 2-ME or DTT. I have to perform immunoprecipitations and
immunohistochemistry with this antibody and therefore have to alkylate the
reduced proteins and reducing agent.
I have two questions
1. Has anyone performed immunoprecipitations and immunohistochemitry on
reduced and alylated proteins.
2. How do you store stock solutions of N-ethyl-maleimide, iodoacetamide
Thanks in advance
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