Storage of alkylation reagents

S.D. Wainwright ogsdw at ssa.bris.ac.uk
Mon Mar 10 09:33:35 EST 1997



Dear All 
  
My antibody only seems to detect its target protein when it is reduced
with 2-ME or DTT.  I have to perform immunoprecipitations and
immunohistochemistry with this antibody and therefore have to alkylate the
reduced proteins and reducing agent.  

I have two questions 

1. Has anyone performed immunoprecipitations and immunohistochemitry on
reduced and alylated proteins.

and 

2. How do you store stock solutions of N-ethyl-maleimide,  iodoacetamide
and iodoacetate.


Thanks in advance

Shane Wainwright
 




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