Fellow Biochemists : Advice needed

Hernan Espinoza espinoza at cgl.ucsf.edu
Mon Mar 10 13:45:32 EST 1997


Howdy,

        A friend of mine is trying to separate out very basic proteins
on polyacrylamide using urea as a denaturant under non-reducing, acidic
conditions.  It has worked before, but not consistently.  He was hoping
to talk to someone who has worked with these kind of gels before so he
can get an idea of where to begin troubleshooting and optimization.
Any advice, references, and/or pointers to an expert welcome.  Thanks
in advance.

                -Hernan



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