how to stop protein degradation?

Levon Fendekian elf8365 at umoncton.ca
Mon Mar 10 21:40:15 EST 1997


XiaoYin Zhong <medm049 at uabdpo.dpo.uab.edu> wrote:

>Dear Dr. protein:
>
>I cloned a protein with His-tag at C-end. After I purified this perotein
>by Ni-column, SDS-PAGE gel show that the protein was degraded.What is
>the reason? Is there any other methods which can solve this problem
>except using  proteinase inhibiter coke-tail or EDTA in the buffer?
>
>Thank you for your help!
>
>
>
>
>qiu at orion.cmc.uab.edu

Are you working at 4 C ?




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