1 mM Tris?

Stephen P. Driska PhD driska at astro.ocis.temple.edu
Mon Mar 10 14:43:56 EST 1997


hbkg at hitel.kol.co.kr wrote:
: We tried to dissolve DTNB, CAT substrate, in Tris buffer, and
: found that DTNB was not dissolved in Tris buffer at all, which
: was very strange. We changed water in vain.
: We found at last the mistake of using 1mM Tris instead of appropriate
: concentration. 
: I am curious HOW TRIS AFFECTS DTNB SOLUBILITY.

	What is the pH of the solution?  1 mM is not a very high concen-
tration and may not hold the pH to a pre-set value if you add a significant
amount of a solute that may be acidic or basic.  
	Good luck, someone should be able to help you in this group.

			Steve

--
Steve Driska, Physiology Department, Temple University Medical School
Philadelphia, PA 19140 USA                  (215) 707-3283 
driska at astro.ocis.temple.edu  




More information about the Proteins mailing list