Q. About Protein Expression !!!
Miss ELLA CHAN
boella at uxmail.ust.hk
Tue Mar 11 11:39:06 EST 1997
Could somebody kindly tell me at which OD600 will be the optimum
condition for the induction of protein expression by using IPTG
in bacterial strain ?
I have been heard that we can start the induction at OD600=0.6, that
is the pre-log phase of the growing bacteria or we can do it at
OD600=1.5 which is the late log phase. Which condition should be the
Thanks so much for your help !
boella at uststu.ust.hk
More information about the Proteins