Isoenzymes separation

Matteo Antorini matteo.antorini at bc.biol.ethz.ch
Fri Mar 14 12:48:51 EST 1997


Hi all,

I need to isolate different isoenzymes, which are of similar isoelectric
point. I tried to do it by preparative isoelectric focusing, but I can't
get satisfactory results suitable for crystallographic studies. 
 
I know of  two products that might interest me: chromatofocusing columns
and Isoprime (Multi-chambered Electrofocusing unit), both  from
Pharmacia.

Any experience report with those or even other preparative isoelectric
focusing techniques will be appreciated. 
 

Thank you in andvance

Matteo


-- 
Matteo Antorini    
_______________________________________ _/_/_/_/_/_/_/  _/  Swiss
mailto:matteo.antorini at bc.biol.ethz.ch _/      _/  _/  _/  Federal
phone: 0041-1-632-3020                _/_/_/  _/  _/_/_/  Institute
phone (priv): 0041-1-850-5367        _/      _/  _/  _/  of
Fax: 0041-1-632-1121                _/_/_/  _/  _/  _/  Technology
__________________________________________________________________



More information about the Proteins mailing list