Q. About Protein Expression !!!

Cheng Luo cheng at SUN.FYSBOT.UMU.SE
Tue Mar 25 04:36:36 EST 1997



It very much depends on the insert. The protein which you
expressed, generally O.D 0.4-0.6 is applied, and O.D=1.5 also works well
for some induction. The concentration of IPTG is another important factor,
some need higher, some need lower, some even don't need at all(IPTG reduce
the expression), That is why some need a LysS plasmid which carries the
T7lysozme to partially digest T7RNA polymerase. These attribute to the
different protein structres which may be compatible, or may be not in
E.coli.  Hope this helps! Cheng


On Tue, 25 Mar 1997, Cheng Luo wrote:

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> From: boella at uxmail.ust.hk (Miss ELLA CHAN)
> Newsgroups: bionet.molbio.proteins
> Subject: Q. About Protein Expression !!!
> Date: 11 Mar 1997 16:20:01 GMT
> Organization: Hong Kong University of Science and Technology
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> 
>   Hi,
> 
>   Could somebody kindly tell me what is the optimum OD600 for the induction
>   of protein expression in bacterial strain using IPTG?  
> 
>   I have been heard that we can start the induction with OD600 = 0.6
>   ie. the pre-log-phase or OD600 = 1.5 which is at the late log-phase.
>   Which condition should be the best ??
> 
>   Thanks for your help !
> 
>    Regards,
>    Ella
> 
>  
> 
> --
> $***********************************************************************$
> $Dr. Cheng Luo			   Tel.  46 90 165474			$
> $Dept. of Plant Physiology	   Fax   46 90 166676			$
> $University of Ume		   Email: cheng.luo at plantphys.umu.se    $
> $90187 Ume			   http://www.plantphys.umu.se/~cheng   $
> $Sweden				  					$
> $***********************************************************************$
> 
> 
> 




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