Affinity Chromatography: Streptavidin agarose

Garfield user at garfield.neuro.ruhr-uni-bochum.de
Tue Mar 18 06:59:02 EST 1997


Hi!

I hybridisize biotinylated oligonucleotides to a specific RNA. Then i coupled 
it to streptavdin agarose (SIGMA). After loading with proteins i have some 
problems with unspecific binding to streptavidin agarose.

How can i prevent this? Whats about BSA? Can i use this?

-----
Dr. R. Trippe
Ruhr-University Bochum, Germany

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