Affinity Chromatography: Streptavidin agarose
user at garfield.neuro.ruhr-uni-bochum.de
Tue Mar 18 06:59:02 EST 1997
I hybridisize biotinylated oligonucleotides to a specific RNA. Then i coupled
it to streptavdin agarose (SIGMA). After loading with proteins i have some
problems with unspecific binding to streptavidin agarose.
How can i prevent this? Whats about BSA? Can i use this?
Dr. R. Trippe
Ruhr-University Bochum, Germany
Please don't reply with email!
More information about the Proteins