need some help with giant protein

THE GREEK pxpst2 at vms.cis.pitt.edu
Wed Mar 26 15:17:59 EST 1997


In article <199703241409.GAA18637 at net.bio.net>, margajulia at tresnet.com
("Margarita Julia-Sape") wrote:

/> Hi, netters!
/> I am trying to study titin, a giant protein of about 3 MDa. I would like to
/> know how to prepare a 2% acrylamide gel. And from which sources could I
/> obtain different MAbs against it?
/> Thanks. 

I would think that an agarose gel might be a better choice.  A 2% PA-Gel
would not hold together.
Also Gel filtration would be another choice
Peter Pediaditakis



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