Smeary 2-D protein gels - help!!

Thomas N. Dentel tndent at planetx.bloomu.edu
Tue Mar 25 21:02:03 EST 1997


On Wed, 19 Mar 1997, Aine L. Plant wrote:
 
> We have been running 2-D gels for approx. 2 years using the protean 2-D
> gel system.  Up until now the gels have been running well, however,
> recently we have started to periodically obtain gels that have
> substantial amounts of smearing.  This happens about every 20 gel runs
> and appears to be a problem with the SDS-PAGE rather than the ief since 
> 1)  it only applies to a few tubes out of the total run for ief and 2)
> the markers are also smeared.  We have checked our solutions and are
> careful when casting gels.  
> 
	I am hardly an expert, just getting into electrophoresis myself,
but I have recent (like today) experience with smeared gels. My experience
is that if you have cellular crud in the homogenate that gets onto the
wickes (assuming your set up uses wicks) you get lots of smearing.


	T.N. Dentel
	tndent at planetx.bloomu.edu
	Biology Dept.
	Bloomsburg University
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