need some help with giant protein

Bernard Heymann heymann at ubaclu.unibas.ch
Wed Mar 26 14:38:30 EST 1997


In article <199703241409.GAA18637 at net.bio.net>, margajulia at tresnet.com
("Margarita Julia-Sape") wrote:

> Hi, netters!
> I am trying to study titin, a giant protein of about 3 MDa. I would like to
> know how to prepare a 2% acrylamide gel. And from which sources could I
> obtain different MAbs against it?
> Thanks. 

Why don't you try agarose gels?  Unfortunately I don't have a protocol for
proteins.

Good luck.

-- 
Bernard Heymann
M. E. Müller Institut, Biozentrum
Universität Basel
CH-4056 Basel
Switzerland
heymann at ubaclu.unibas.ch



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