getting rid of hemoglobin

Dora Berman dberman at UMABNET.AB.UMD.EDU
Fri May 2 16:10:28 EST 1997


I prepare cytosolic extracts from chunks of fat containing some blood 
vessels.  I need to get rid of the hemoglobin present in the extracts in 
order to assay for the activity and expression of lipolytic enzymes in 
the extract.  I will appreciate any input on how to selectively remove 
the hemoglobin from my extracts.

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