Structure of His-Tag complex
Thorsten.Schmidt at rz.ruhr-uni-bochum.de
Thu May 8 07:57:27 EST 1997
The His-Tag-System is widely used for protein purification.
(Novagene, Quiagen ...)
Using a special expression vector, a "His-Tag", a repeat of six Histidines,
is added to the target protein.
A special resin is then loaded with nickel-ions.
When one loads the column with a protein mixture only
the target protein forms with its histidines a complex with the nickel and
After washing steps the protein can be eluted with a buffer containing
I want to know the exact structure of this complex and the reason, why
it is possible to elute the protein with Imidazole.
Can you help me, perhaps tell me a reference?
Thank you very much in advance for your answer
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