Buffer for lyophylization
Lipid Research Laboratory
rlax at erols.com
Sat May 10 15:43:37 EST 1997
Michael DiDonato wrote:
> I am wondering if anyone knows which is the best buffer to lyophylize from?
> Excluding water, what buffer would leave beind the least amount of salt
> upon lyophylization?
> Any suggestions are extremely appreciated.
> Michael DiDonato
> Department of Biochemistry Research
> Hospital for Sick Children
> Toronto, Ontario, Canada
> michael.didonato at utoronto.ca
I use routinely ammonium bicarbonate. Before the lyophilization step the
protein sample is dialyzed against ammonium bicarbonate (25 mM usually).
Also, I add 0.1% 2-mercaptoethanol in the buffer.
George Washington University
Lipid Research - VA medical Center
50 Irving Street NW
Washington, DC 20422
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