Buffer for lyophylization

Mark Dalton mdalton at worldnet.att.net
Sat May 10 07:47:42 EST 1997

I would use amonium acetate or am. carbonate at 50mM if you wish to 
totally get rid of the salt by mult. lyopholyzation steps. Just keep 
adding water and refreezing and relyopholyze the amonium acetate/carb 
will disipate after two or three cycles.
Michael DiDonato wrote:
> I am wondering if anyone knows which is the best buffer to lyophylize from?
> Excluding water, what buffer would leave beind the least amount of salt
> upon lyophylization?
> Any suggestions are extremely appreciated.
> Mike
> --
> Michael DiDonato
> Department of Biochemistry Research
> Hospital for Sick Children
> Toronto, Ontario, Canada
> michael.didonato at utoronto.ca

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