Protein Identification

[Cornelius Krasel]

Gene S. Hall (hall at RUTCHEM.RUTGERS.EDU) wrote:
> What is the basic way to absolutely determine an unknown protein?  We
> are using SEC to separate human proteins from plasma.  How can we 
            ^^^size exclusion chromatography?

> absolutely determine the molecular weight of these proteins and identify
> them.

To determine the molecular weight:
- crude estimate: SDS polyacrylamide electrophoresis
- precise determination: mass spectroscopy

To identify proteins:
- antibodies on western blot
- N-terminal sequencing (or sequencing of -- e.g. tryptic -- peptides)

--Cornelius.

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/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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