Protein Identification

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Mon May 12 03:04:24 EST 1997


Gene S. Hall (hall at RUTCHEM.RUTGERS.EDU) wrote:
> What is the basic way to absolutely determine an unknown protein?  We
> are using SEC to separate human proteins from plasma.  How can we 
            ^^^size exclusion chromatography?

> absolutely determine the molecular weight of these proteins and identify
> them.

To determine the molecular weight:
- crude estimate: SDS polyacrylamide electrophoresis
- precise determination: mass spectroscopy

To identify proteins:
- antibodies on western blot
- N-terminal sequencing (or sequencing of -- e.g. tryptic -- peptides)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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