TCA and HIC protein pufn

Ian McFarlane I.McFarlane at icrf.icnet.uk
Mon May 12 10:49:26 EST 1997


Hi,

My protein of interest happens to be soluble in 10% TCA. I am going to use
TCA as the first step in its pufn. Does anyone know if I can go on to do
hydrophobic interaction chromatography without dialysing away the TCA.
Obviously anion or cation exchange would require dialysis. Any
suggestions?

Ian Mc



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