Buffer for lyophylization
mbonifac at mail.usyd.edu.au
Thu May 15 06:41:34 EST 1997
Michael DiDonato <michael.didonato at utoronto.ca> wrote in article
<michael.didonato-ya02408000R0905971112440001 at resunix.ri.sickkids.on.ca>...
> I am wondering if anyone knows which is the best buffer to lyophylize
> Excluding water, what buffer would leave beind the least amount of salt
> upon lyophylization?
> Any suggestions are extremely appreciated.
> Michael DiDonato
> Department of Biochemistry Research
> Hospital for Sick Children
> Toronto, Ontario, Canada
> michael.didonato at utoronto.ca
Bicarb Buffers are the go!!! The buffers, due to the partial pressures,
blow off as CO2 and H2O from the sample on freeze drying.
Regards Michael Bonifacio
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