Buffer for lyophylization

Martin Offterdinger a8803349 at unet.univie.ac.at
Thu May 15 07:28:13 EST 1997


On 15 May 1997 11:41:34 GMT, "Michael Bonifacio"
<mbonifac at mail.usyd.edu.au> wrote:

>
>
>Michael DiDonato <michael.didonato at utoronto.ca> wrote in article
><michael.didonato-ya02408000R0905971112440001 at resunix.ri.sickkids.on.ca>...
>> 
>> I am wondering if anyone knows which is the best buffer to lyophylize
>from? 
>> Excluding water, what buffer would leave beind the least amount of salt
>> upon lyophylization?
>> 
>> Any suggestions are extremely appreciated.
>> 
>> Mike
>> 
>> -- 
>> Michael DiDonato
>> Department of Biochemistry Research
>> Hospital for Sick Children
>> Toronto, Ontario, Canada
>> michael.didonato at utoronto.ca
>
>
>
>Bicarb Buffers are the go!!! The buffers, due to the partial pressures,
>blow off as CO2 and H2O from the sample on freeze drying.
>
>Regards Michael Bonifacio
>
>
>
>
>> 
Ammoniumcarbonate will do the same but puffer inthe neutral range.
Martin




More information about the Proteins mailing list