Prove for cell fractionation?

Dr E. Buxbaum EB15 at le.ac.uk
Mon May 19 08:15:15 EST 1997


"Thorsten Schmidt" <Thorsten.Schmidt at rz.ruhr-uni-bochum.de> wrote:
>Dear reader!
>
>I made a fractionated protein isolation of cells and got
>fractions that are told to be 
>1. "nuclear"
>2. "mitochondrial/synaptosomal"
>3. "microsomal", 
>4. "cytosolic",
>But how can I prove that they really contain that and that perhaps
>no other fractions contains mitochondrial proteins?
>
>I´ve been told that one uses
>citrate synthase, lactate dehydrogenase and choline phosphotransferase
>as "marker enzymes" but how?
>Can you tell me special assays for them?
>There are no antibodies availible which detect them!

The first thing you should do is to get a book on basic cell biology. 


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