Q: BrCN-cleavage in a gel?

[room 103]

Hi there,

I am interested in a method to cleave a protein with a molecular mass >300
kDa with BrCN. Enzymatic digests would lead to more than 400 fragments,
therefore I think BrCN might be the best choice. Although I could blot and
cleave the protein on PVDF-membrane, I wonder whether it wouldn't be a
better option to cleave it in the gel and elute the peptides. 
Can anybody provide me with a method/literature for cleavage in the gel?
Suggestions for an alternative approach would also be appreciated.

Thanks in advance,

Peter

p.weber at bham.ac.uk