Insoluble GST-fusion protein

Uwe Klein uwe at itsa.ucsf.edu
Fri May 23 14:38:34 EST 1997


In article <5lqbqq$h03 at uni00nw.unity.ncsu.edu>, vwbauer at unity.ncsu.edu
(Vernon Wayne Bauer) wrote:

> I need to know what the easiest (and best if they are not the same) 
> method to get an insoluble GST-fusion protein expressed in BL21 cells 
> bound to glutathione beads.  
-------------------

We use the method of Frangioni and Neel with N-Laurylsarcosine and Triton
as detergents. It works quite well.

Frangioni and Neel: Solubilization and Purification of Enzymatically
Active Glutathione-S-Transferase (pGEX) Fusion Proteins, Analytical
Biochemistry 210, 179-187 (1993)

-- 

Uwe Klein, Ph.D.     E-mail uwe at itsa.ucsf.edu
Box 0984-IRE
University of California San Francisco
San Francisco, CA 94143



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