limited proteolysis

Alan Schoenfeld aschoen at aecom.yu.edu
Tue May 27 16:14:46 EST 1997


I want to take a metabolically labeled band from a gel by either slicing
the band out of the gel or by transfering it to a membrane and then do
limited proteolysis (V8, chymotrypsin, etc.) on the gel slice or
membrane.  Can anyone give me a protocol to do this?  If I cut from a
gel, can I dry the gel first (I need to find my band by
autoradiograph)?  Which enzymes are best/cheapest?  Thanks.



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