6M Guanidine HCl and IEC

Peter Hains peter at indy.bio.uts.edu.au
Mon May 26 23:43:01 EST 1997


Hello all,

I was wondering if anybody knows for sure if you can deionise 6M
guanidine HCl for use in ion-exchange chromatography the way you can with
8M urea. I have tried 8M urea but I am not convinced that it fully
denatures the proteins and I know that 6M Gdn HCl does. 

Thanks in advance, Peter.



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