Cannot bump GST-proteins off the GSH-beads

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed May 28 10:44:51 EST 1997


ct133 at columbia.edu wrote:
> I need some help with how to bump off my GST-fusion proteins from the
> GSH-beads. I've tried to bump off with 5, 10 and 15 mM of GSH in 50 mM
> Tris pH 7.5. I've gone up to 20 mM and still couldn't bump the
> proteins off. Is there any trick I can do?

Have you checked the pH after adding the GSH? (GSH is fairly acidic.)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */



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