green at salk.edu
Thu May 29 20:06:07 EST 1997
We seem to have a specificity problem with enterokinase, and wonder if
anyone has any ideas.
We are working with a soluble protein produced in High5 insect cells. The
N-terminus carries an epitope which includes an enterokinase recognition
site (DDDDK). There are (unsurprisingly), no other such sequences in the
polypeptide (~40kDa). When we digest with the enzyme for 5 hours at 4°C,
the protein is cleaved into two fragments at a site clearly different from
the expected N-terminal one.
suggestions? The enterokinase was purchased from Strategene (supposedly
More information about the Proteins