Cannot bump GST-proteins off the GSH-beads

ct133 at columbia.edu ct133 at columbia.edu
Thu May 29 22:02:03 EST 1997


>No guarantees, but check the pH of your elution buffer.  It needs to be
>around 7.6 to work effectively - and as you increase [GSH], you will need
>more buffering capacity.
>
>You could also try at different temperatures - try 5 minutes at room
>temperature (which is about 30C here, so maybe a bit lower (-; ) or 30
>mins at 4C if the pH is fine.

I've checked the pH with pH strip. It was between 7.5 to 8.0. I was
told that's good enough. But may be not. I'll check with pH meter next
time. 

Thanks for the advice.

Chai




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