proteolysis in gel

Nora Plesofsky-Vig npv at MANI.CBS.UMN.EDU
Thu May 29 11:21:13 EST 1997

We sent out a protein that was N-terminally blocked, and it was  
digested with modified sequencing grade trypsin (Boehringer Mannheim)  
in the gel; peptides were separated by HPLC. The reference to this  
procedure which we were given:

Rosenfeld, J., Capdeviell, J., Guillemot, J., and Ferrara, P. (1992)  
Anal. Biochem. 203, 173-179

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