Cannot bump GST-proteins off the GSH-beads

[Richard P Grant]

ct133 at columbia.edu wrote:

} I've checked the pH with pH strip. It was between 7.5 to 8.0. I was
} told that's good enough. But may be not. I'll check with pH meter next
} time. 

Should be good enough - I always use a strip.  Perhaps your protein just
will not come off - some are like that.

R

--
Richard P. Grant  MA  DPhil          University of Oxford
Nuffield Department Obstetrics and Gynaecology      FFPGP
rgrant'at'molbiol.ox.ac.uk            http://www.molbiol.ox.ac.uk/~rgrant
----------------# Set your thermonuclear devices on stun #----------------