Homemade Chemiluminescent Protocols?

David Aldridge aldridge at *NOSPAM*.mbcmr.unimelb.edu.au
Fri May 30 19:06:33 EST 1997


In article <338EFCA7.41C6 at risotto.mit.edu>, Thomas Cameron
<cameron at risotto.mit.edu> wrote:

> Company kits end up costing $3-5 per western blot which is 90% of the
> cost of the whole procedure.
> 
> People must have some good homemade HRP (and AlkPhos) Chemiluminescent
> Detection protocols or references?
> 
> Could you send me one or point me to it?  Thanks.

*Luminol 4mg/ml in DMSO         1ml
*p-iodophenol 1mg/ml in DMSO    1ml
1M Tris.HCL pH 7.5              0.6ml
H2O2 (30%)                      5 microL
H2O                             7.5ml

*Store at -20
Mix this stuff up fresh a few minutes before use.
Works for me!



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