Biorad Protein Standards migrating

liuying at PUBMS.PKU.EDU.CN liuying at PUBMS.PKU.EDU.CN
Mon Nov 3 08:00:56 EST 1997


I think the degrade of the marker is the answer.Try a new marker.

Ying Liu
On 31 Oct 1997, Obaid Yusuf Khan wrote:

> I am using the Biorad Kaleidoscope Prestained Standards to size my proteins 
> on a 12% PAGE gel ("29:1). The markers below 31800 KD are very faint and 
> fuzzy and I am unable to see the 17800 and 7100 KD markers.
> I am using the markers without any heat denaturing (I presume they dont require 
> it).
> Does any one have any tips to offer me on this please.
> Thanks 
> Obaid Khan
> 
> 
> 




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