Ecoli contaminant Histag purification

Luc CAMOIN camoin at cochin.inserm.fr
Wed Nov 5 11:46:52 EST 1997


Dear Netters,

	We tried to purify our His-tagged recombinant protein expressed in
Ecoli using Nickel affinity purification columns. Two proteins were
retained and eluted at 100 mM imidazole. These two proteins were sequenced.
	One was a FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD from
Ecoli. This protein was known to bind Ni with high affinity.
	The second one was a chaperonin protein GROEL from Ecoli. This
protein contains one His and one Cys.
	Has anybody identified already this last proteins in immobilized
metal affinity chromatography?
	Does anyone have any explanation about this binding?


	Thanks in advance

Luc CAMOIN





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