EDTA in lysis buffer?

user at mpi.com user at mpi.com
Wed Nov 5 11:47:49 EST 1997


I've been playing around with conditions for lysis of mammalian cells
lately (for subsequent IP or western analysis), and I'm wondering about
the advantages and disadvantages of including EDTA.  Many people do; many
others don't.  I've heard some stories of protein interactions breaking up
as a result of the chelating properties of EDTA, which I suppose could be
a potential disadvantage if studying specific interactions.  What are the
advantages?  Inhibition of enzymatic activities (like proteases)? 
Cleaning up non-specific interactions?  Any other thoughts?

-HS



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