Biorad Protein Standards migrating

[Harold V. Taylor]

Obaid Yusuf Khan wrote:
> 
> I am using the Biorad Kaleidoscope Prestained Standards to size my proteins
> on a 12% PAGE gel ("29:1).

Dear Obaid,

your problem is that you're using a 12 % PAGE gel. Proteins smaller than
approx. 25 kDa get fuzzy on non-gradient gels. Either try tris-tricine
gels and higher acrylamid concentrations or tris-glycine and gradient
gels.

Have fun, ;-)
 
Harold V. Taylor
Physiol. Chem. Institut
Hoppe-Seyler-Str. 4
D-72076 Tübingen

phone: 07071-297-3353
fax:   07071-29-3361
email: cbiei01 at uni-tuebingen.de