Isoelectric Focusing

D. Vadnais dvadnais at
Wed Nov 5 19:32:57 EST 1997

I am going to use IEF slab gels from Bio-Rad to separate invertase 
isozymes from alfalfa.  I have never done this procedure before, and I am 
not really sure of the protocol.  I have only the protocols that are used 
for 2D gel electrophoresis using the capillary tubes.  I need to know 
what buffers I should be using to run these gels, and what type of 
loading buffer I require for my samples.  
Thanks in advance for any information you can give me.

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