Isoelectric Focusing

Dean.Sequera at Dean.Sequera at
Thu Nov 6 12:13:39 EST 1997

In article <63r37p$cgv at>,
  dvadnais at (D. Vadnais) wrote:
> I am going to use IEF slab gels from Bio-Rad to separate invertase
> isozymes from alfalfa.  I have never done this procedure before, and I am
> not really sure of the protocol.  I have only the protocols that are used
> for 2D gel electrophoresis using the capillary tubes.  I need to know
> what buffers I should be using to run these gels, and what type of
> loading buffer I require for my samples.
> Thanks in advance for any information you can give me.
> Dave

I suggest you contact our offices at Pharmacia Biotech to get a free copy
of the Hoefer Protein Electrophoresis Applications Guide. It is complete
with theory, practice, pictures, illustrations, etc. Isoelectric focusing
is covered in extensive detail. Contact: Pharmacia Biotech Inc. 500,
Morgan Boulevard Baie d'Urfe Quebec H9X 3V1


Ask for Marie-France Belanger, the electrophoresis specialist in your

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