p-Nitrophenol Protease Substrates?

Mark A. Blight blight at igmors.u-psud.fr
Thu Nov 13 06:26:19 EST 1997


I am trying to characterize a novel protease secreted by a bacterium.
Could anyone help me with the following questions please:

(i) What kind of tests must I do to characterize the type of protease by
its cleavage site? i.e. serine protease etc.

(ii) Do you know of any "general" p-nitrophenol protease substrates that
I can use to biochemically characterise the protease? (temperature, pH,
salt dependence etc). If not, what about any "specific" substrates for
other proteases that I could try?

Thanks for any help you can give.

Please reply by e-mail if poss.

Dr. Mark A. Blight,
Institut de Génétique et Microbiologie,
Université de Paris Sud,
Bâtiment 409,
91405 Orsay,

Tel:      +33 1 69156699
Fax:      +33 1 69157808
e-mail:   blight at igmors.u-psud.fr
Internet: http://www.igmors.u-psud.fr/holland/holland-home-page-gb.html

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