TCA precipitation of proteins

Alexis Gautreau gautreau at curie.fr
Sun Nov 16 15:38:59 EST 1997


Jens Kondrup wrote:
> 
> Is TCA better than PCA?
> Jens Kondrup
 Hi,
For precipitating proteins, just in order to reduce the volume, before a
blot. I use a very simple protocol:
Just complete the lysate up to 85%acetone
spin 800g 5min (no fast zizz, as proteins precipitate stick to the tube)
Dry the pellet 5 min at RT 
And resuspend in SDS-Loading buffer.
Thats it!
Alexis Gautreau
-- 
ÐÏࡱá



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