protein staining problems

Dr E. Buxbaum EB15 at le.ac.uk
Thu Nov 20 14:12:31 EST 1997


G Garcia-asua wrote:
> 
> Why can't I stain my protein with coomasie, silver or anything else
> I've tried? Any help?

A little bit more information would be needed to answer your question.
What is your sample and how was it treated? Presumably you are talking
about the staining of proteins in gels after electrophoresis. Which
method did you use here: Laemli, Weber-Osborn, Lichtner-Wolf...? Round
gels, large flat gels, minigels? Which staining methods did you use? For
silver staining alone there must be dozens of recepies.



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