Protein, RNA and Lipid Separation

Brett Phinney brettsp at mail.urtexas.edu
Thu Nov 20 10:08:35 EST 1997


I'm working with a membrane containing virus and I was wondering if
there was an easy way to separate the membrane proteins from the
membrane lipids and the viruses RNA.

As a side question, when I load whole virus on a SDS-PAGE gel, I can get

a good separation of the proteins I am looking for. My question is, what

happens to the RNA and Lipids that get loaded onto the gel? Do they run
through the gel, get stuck at the top, or complex with my proteins
somehow?

Thanks a lot in advance

Brett






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