Assay for allosteric enzymes

moreaut at moreaut at
Fri Nov 21 04:04:45 EST 1997

dear readers,

I would like to setup a one-day practical session related to allosteric
enzyme kinetics for bachelor's degree students. The enzyme they were
studying up to now was rabbit phosphorylase b but I am not satisfied with
the assay. Then I am looking for another allosteric enzyme to study, 
preferentially commercially available and assayed with a photometric procedure.
I was thinking to phosphofructokinase or ATC (Aspartate Trans Carbamylase) but
I have no idea if this system is appropriate.

Any ideas or a detailed protocol are welcomed.

Thank you very much in advance for your assistance.

Thierry Moreau

|  Dr. Thierry MOREAU                                                       |
|  Laboratoire d'Enzymologie et Chimie des Proteines                        |
|  CNRS EP 117                         Phone: (33) 02 47 36 61 77           |
|  2bis, Bd Tonnelle                   Fax:   (33) 02 47 36 60 46           |
|  37032 TOURS cedex  FRANCE           Email: moreaut at         |
|                                                                           |
|  Please visit PROLYSIS at:      |

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