meton at rci.rutgers.edu
Sun Nov 23 16:52:47 EST 1997
soumkr at ki.se (Souren Mkrtchian) writes:
>Does anyone know any in vitro protein refolding assays? The goal is to
>assess impact of possible cochaperones on the protein folding
>capabilities of BiP (HSP70 of endoplasmic reticulum).
I have just read the following article, "Chaperonin-mediated Folding of Green Fluorescent Protein" by Y. Makino et al. in the JBC vol. 272. No. 19 pp 12468-12474. The approach was to denature GFP and monitor its refolding in the presence of GroEL. This method might allow you to develop an assay for your experiments.
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