protein staining problems

Thomas Hettmann hettmann at physik.mu-luebeck.de
Tue Nov 25 11:36:20 EST 1997


G Garcia-asua wrote:
> 
> Why can't I stain my protein with coomasie, silver or anything else
> I've tried? Any help?
> 
> Will Garcia-Asua

I suppose you are talking about staining of Laemmli-gels after
electrophoresis.
I had some difficulties in staining my protein, and it turned out that
it is highly glycosylated. Maybe you should try a stain for sugar
residues as well? - Just an idea...

Greetings, Thomas
-- 
Thomas Hettmann - Institut fuer Biochemie 
Medizinische Universitaet zu Luebeck     
Ratzeburger Allee 160                    
23538 Luebeck                            
Fax: +49-451-500-4068                
E-Mail: hettmann at physik.mu-luebeck.de



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