Protein assay for membrane proteins

Keith Jolley K.Jolley at soton.ac.uk
Wed Nov 26 11:43:40 EST 1997


Just a small follow-up.  You can incubate the BCA reaction at room
temperature or at 37 deg C.  You just need to incubate for longer.  We
routinely use 96-well plates for this.

On Tue, 25 Nov 1997 09:56:28 -0800, "Dr E. Buxbaum" <EB15 at le.ac.uk>
wrote:
>3) The BCA method: Can be quite sensitive. Requires incubation at 65
>degrees, so that 96 well plates can not be used. Serious interference by
>reducing substances, however, non-reducing detergents have little
>effect.

-- 
Dr. Keith Jolley
Dept. of Molecular Microbiology
Southampton General Hospital, UK
Tel: 01703-798895  Fax: 01703-774316



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