covalent coupling strategies

Rod Macfarlan rmacfarl at csl.com.au
Thu Nov 27 18:44:17 EST 1997


I am looking for methods to couple synthetic peptides to a carrier
protein, ideally through lysine side chains. My problem is that the
protein is in 8M urea. Is there a way of doing this, and if not, does
anyone have a suggestion for an alternative approach (no thiols
available - but thiolation might be possible).

Rod Macfarlan



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