Concentrating Membrane Proteins
federici at icgm.cochin.inserm.fr
Wed Sep 10 02:10:43 EST 1997
In article (Dans l'article)
<19970901030801.XAA01638 at ladder02.news.aol.com>, rsjolund at aol.com
(RSjolund) wrote (écrivait) :
> I've got a 32 kDa protein from a plant cell plasma membrane. It is
> extracted well in a medium containing CHAPS, but if I precipitate it with
> ammonium sulphate (40% saturation) if does NOT go back into solution.
> What are some good techniques for concentrating the protein (prior to 2D
> gels). The goal is to sequence the protein.
> Dick Sjolund
> Biological Sciences
> Univ. of Iowa
> r-sjolund at uiowa.edu
Have you tried filtration upon membrane, using concentrators of Amicon?
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