How to deglycosylate a protein?
Thorsten.Schmidt at rz.ruhr-uni-bochum.de
Thu Sep 11 15:59:26 EST 1997
I examine a protein which I can detect by Western Blots.
It is detected at a higher molecular wight than expected according to
the aminoacid sequence.
I now want to test whether it is glycosylated or not!
How can I do this? What do I need?
Are there enzymes/kits availible for deglycosylation?
Are there different types of protein glycolysation and can I examine
them all just with one test?
Can I conclude protein glycosylation just from special aminoacid sequences?
It would be too kind if you´ll send me an answer
Thank you so much in advance
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